Protocol - Competent cells 

Protocol - Transformation of E. coli

Protocol - Electroporation

Protocol - Calcium phosphate transfection

Recipe for 300 mOsm solution: 9.448 g  NaCl / l = 161.7 mM NaCl

Recipe for tricaine solution: 1.5 g 3-aminobenzoic. acid ethyl ester (tricaine) in 1 l of tap water

Recipe for CM buffer: 100 mM CaCl₂, 100 mM MgCl₂

Ricetta per il brodo

How to extract Cm, Rs, Cm from a voltage pulse

On line tool to calculate Rs, Rm and Cm: Rs Calculator

Pusch Lab page