Dr. Armando CARPANETO

Istituto di Biofisica
Consiglio Nazionale delle Ricerche

Via De Marini 6, 16149 Genova, Italy
Tel: +39010-6475559
Fax: +39010-6475500
Email: carpaneto@ge.ibf.cnr.it

Short curriculum vitae et studiorum
-Doctoral degree in Physics at the University of Genoa (grading 110/110 cum laude).

- Jan. 1993-July 2001 fellowship at Cybernetics and Biophysics Institute (ICB), Research National Council of Italy (CNR).
- Aug. 2001 current permanent position: researcher at the Institute of Biophysics of Italy’s CNR.
- Academic years 2003-2007: lecturer in "Plant physiology" within the Biology course at the Genoa University.

-Winner of the Antonio Borsellino Award (by the Italian Society for Pure and Applied Biophysics) in 1994 for his graduation thesis.
-Research fellow of the Alexander von Humboldt Foundation.
- Visiting scientist for 18 months at the German University of Wuerzburg collaborating with Prof. R. Hedrich.

-Carpaneto has been involved in a series of national and european projects as researcher and tutor of PhD students and postdocs.

-Carpaneto was an invited speaker to more than 10 national and international meetings., attended 18 national and international congresses, published 29 abstracts in the proceedings of international congresses, 27 abstract in the proceedings of national congresses and has 6 publications in national journals/ books.


Research topics
Carpaneto’s research efforts have been devoted to the investigation of the molecular mechanisms controlling ion transport through plant/animal plasma/intracellular membranes using electrophysiological, biophysico-chemical and wide-field fluorescence microscopy techniques

Publications in refereed international journals
1) F. Gambale, A.M. Cantù, A. Carpaneto and B. Keller.
Fast and slow activation of voltage dependent cation channels in radish vacuoles.
Biophysical Journal (1993), 65: 1837-1843.

2) A. Carpaneto, A.M. Cantù, H. Busch and F. Gambale.
Ion channels in the vacuoles of the seagrass Posidonia oceanica.
FEBS Letters (1997), 412: 236-240.

3) M. Bregante, A. Carpaneto, F. Pastorino and F. Gambale.
Effects of mono- and multi-valent cations on the inward-rectifying potassium channel in isolated protoplasts from maize roots.
European Biophysics Journal (1997), 26: 381-391.

4) A. Carpaneto, A. Accardi, M. Pisciotta and F. Gambale.
Chloride channels activated by hypotonicity in N2A neuroblastoma cell line.
Experimental Brain Research (1999), 124: 193-199.

5) A. Carpaneto, A.M. Cantù and F. Gambale.
Redox agents regulate ion channel activity in vacuoles from higher plant cells.
FEBS Letters (1999), 442: 129-132.

6) A. Carpaneto.
A cyclic model for bimodal activation of calcium activated potassium channels in radish vacuoles.
Rivista di Biologia / Biology Forum (2001), 1: 83-104.

7) A. Carpaneto, A.M. Cantù and F. Gambale.
Effects of cytoplasmic magnesium on slowly activating channels in isolated vacuoles of Beta vulgaris.
Planta (2001), 213: 457-468.

8) A. Paganetto, A. Carpaneto and F. Gambale.
Ion transport and metal sensitivity of vacuolar channels from the roots of the aquatic plant Eichhornia crassipes.
Plant, Cell & Environment (2001), 24: 1329-1336.

9) E. Zocchi, A. Carpaneto, C. Cerrano, G. Bavestrello, M. Giovine, S. Bruzzone, L. Guida, L. Franco and C. Usai.
The temperature-signaling cascade in sponges involves a heat-gated cation channel, abscisic acid and cyclic ADP-ribose.
Proc. Natl. Acad. Sci. USA (2001), 98:14859-14864

10) A. Carpaneto, M. Dalla Serra, G. Menestrina, V. Fogliano and F. Gambale.
The phytotoxic lipodepsipeptide syringopeptin 25A from Pseudomonas syringae pv syringae forms ion channels in sugar beet vacuoles.
The Journal of Membrane Biology, (2002) 188: 1-12.

11) E. Zocchi, G. Basile, C. Cerrano, G. Bavestrello, M. Giovine, S. Bruzzone, L. Guida, A. Carpaneto, R. Magrassi and C. Usai. ABA- and cADPR-mediated effects on respiration and filtration downstream of the temperature-signaling cascade in sponges. J. Cell Sci. (2003) 116: 629-636

12) A. Carpaneto, R. Magrassi, E. Zocchi, C. Cerrano and C. Usai. Patch-clamp recordings in isolated sponge (Axinella polypoides) cells. Journal of Biochemical and Biophysical Methods. (2003) 55: 179-189

13) A. Carpaneto. Nickel inhibits the slowly activating channels of radish vacuoles. European Biophysics Journal (2003) 32: 60-66

14) A. Carpaneto, A. Paganetto, L. Cornara, E. Pesce, A. Naso and F. Gambale.
Electrophysiological characterization of ion channels in the protoplasts of the Mediterranean seagrass Posidonia oceanica.
Plant, Cell & Environment (2004), 24: 1329-1336.

15) A. Costa, A. Carpaneto, S. Varotto, E. Formentin, O. Marin, E. Barizza, M. Terzi,
F. Gambale and F. Lo Schiavo
Potassium and carrot embryogenesis: are K+ channels necessary for development? Plant Molecular Biology (2004) 54: 837-852

16) J. Scholz-Starke, A. De Angeli, C. Ferraretto, S. Paluzzi, F. Gambale and A. Carpaneto
Redox-dependent modulation of the carrot SV channel by cytosolic pH.
FEBS Letters (2004) 576: 449-454

17) D. Becker, D. Geiger, M. Dunkel, A. Roller, A. Bertl, A. Latz, A. Carpaneto, P. Dietrich, M. R. Roelfsema, C. Voelker, D. Schmidt, B. Mueller-Roeber, K. Czempinski and R. Hedrich.
AtTPK4, an Arabidopsis open-rectifying K+ channel, poised to control the pollen membrane voltage in a pH- and Ca2+ -dependent manner
Proc. Natl. Acad. Sci. USA. (2004) 101(44):15621-6

18) J. Scholz-Starke, F. Gambale and A. Carpaneto
Modulation of plant ion channels by oxidizing and reducing agents.
Archives of Biochemistry and Biophysics (2005) 434:43-50

19) A. Carpaneto, D. Geiger, E. Bamberg, N. Sauer, J. Fromm and R. Hedrich.
Phloem-localized, proton-coupled sucrose carrier ZmSUT1 mediates sucrose efflux under control of sucrose gradient and pmf.
Journal of Biological Chemistry (2005) 280(22):21437-43

20) F. Poree, K. Wulfetange, A. Naso, A. Carpaneto, A. Roller, G. Natura, A. Bertl, H. Sentenac, J.B. Thibaud and I. Dreyer.
Plant K(in) and K(out) channels: Approaching the trait of opposite rectification by analyzing more than 250 KAT1-SKOR chimeras.
Biochem. Biophys. Res. Commun. (2005) 332(2):465-73

21) J. Scholz-Starke, A. Naso and A. Carpaneto.
A perspective on the Slow Vacuolar (SV) channel in vacuoles from higher plant cells.
J. Chem. Inf. Model. (2005), 45, 1502-1506

22) J. Scholz-Starke, A. Carpaneto and F. Gambale.
On the interaction of neomycin with the slow vacuolar channel of Arabidopsis thaliana.
Journal of General Physiology (2006), 127, 329—340.

23) A. Carpaneto, N. Ivashikina, E. Krol, E. Jeworutzki and R. Hedrich.
Membrane-delimited response of Arabidopsis mesophyll cells to cold is triggered via a calcium-permeable cation channel.
Plant Physiology (2007) 143: 487-94.

24) M. Bregante, Y. Yang, E. Formentin, A. Carpaneto, J. Schroeder, F. Gambale, F. Lo Schiavo, A. Costa. KDC1, a carrot Shaker-like potassium channel, reveals its role as a silent regulatory subunit when expressed in plant cells. Plant Mol Biol. (2008) 66:61-72

Chapters in international books
1) A. Carpaneto and F. Gambale.
Voltage-dependent potassium channels in the plasma membrane of higher plant cells.
In "Biophysical Processes in Living Systems" (2001)
Editor: P. Pardha Saradhi
Science Publishers, Inc, Enfield (NH), USA, pp: 191-206

2) M. Dalla Serra, G. Menestrina, A. Carpaneto, F. Gambale, V. Fogliano and A. Ballio.
Molecular mechanisms of action of syringopeptins, antifungal peptides from Pseudomonas syringae pv syringae. (2003)
Eds: Menestrina, G; Dalla Serra, M; Lazarovici, P. (Series Ed: Lazarovici,P. Cellular and Molecular Mechanisms of Toxin Action.). Taylor & Francis Group, London, UK, Vol. 5, pp: 272-295.

3) G. Menestrina, M. Coraiola, V. Fogliano, A. Fiore, I. Grgurina, A. Carpaneto, F. Gambale and M. Dalla Serra.
Antimicrobial lipodepsipeptides from Pseudomonas spp: a comparison of their activity on model membranes. (2003)
Ed: Iacobellis, SN, Kluwer, (in press)